Internucleosomal cleavage of DNA is insufficient evidence to conclude that cell death is apoptotic [letter; comment]
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چکیده
منابع مشابه
Internucleosomal cleavage of DNA is insufficient evidence to conclude that cell death is apoptotic.
In a recent volume of Blood, Yuan et a l l demonstrated internucleosomal cleavage of DNA in erythroid precursors obtained from the bone marrow (BM) of a patient with @-thalassemia major, “lesser” (but unquantitated) amounts of intemucleosomal cleavage in those from a @-thalassemia trait donor, and no evidence of such cleavage in erythroid precursors separated from normal BM. Although no other e...
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Serum-free PC12 cell cultures have been used to study the mechanisms of neuronal death after neurotrophic factor deprivation. We previously reported that PC12 cells undergo "apoptotic" internucleosomal DNA cleavage after withdrawal of trophic support. Here, we have used a sensitive method to detect PC12 cell DNA fragmentation within three hrs of serum removal and have exploited this assay to ex...
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Diphtheria toxin (DTx) is an extremely potent inhibitor of protein synthesis. Cell death has been generally accepted as a straightforward effect of translation inhibition. Using human U937 cells, we found that DTx intoxication leads to cytolysis; indeed, release of 51Cr- and 75Se-labeled proteins could be detected within 7 h. However, little or no cell lysis was observed over a 20-50-h period w...
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Zerumbone is a cytotoxic compound isolated from the herbal plant, Zingiber zerumbet Smith, which exhibits antitumor activity [1-2], anti-inflammatory effects and possesses anti-proliferative potentials in a variety of cell lines [3-4]. DNA fragmentation indicates an early event of apoptosis leading to cell death due to the absence of new cellular proteins synthesizing for cell survival. Previou...
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The Disease Control Priorities (DCP) project has substantially influenced national and global health priorities since 1993. DCP’s basic framework involves identification of disease burdens based on premature deaths and disability and application of the most cost-effective interventions to the largest burdens, taking into account local feasibility. The future impact of DCP will need to take into...
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ژورنال
عنوان ژورنال: Blood
سال: 1994
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v83.7.2005b.2005b